EverGreen™️ mRNA (6x BRIGHTER THAN EGFP)
Available in 3 options:
Naked mRNA (price displayed below)
mRNA + LNP (ready to transfect)*
mRNA + LNP mixture (DIY assembly of LNPs)*
*For pricing contact hello@messenger.bio
CATALOG #
EVERG50U: 50 µg EverGreen™️ mRNA (unmodified)
EVERG100U: 100 µg EverGreen™️ mRNA (unmodified)
EVERG500U: 500 µg EverGreen™️ mRNA unmodified)
EVERG1000U: 1 mg EverGreen™️ mRNA (unmodified)
EVERG50P: 50 µg EverGreen™️ mRNA (N1-methylpseudouridine)
EVERG100P: 100 µg EverGreen™️ mRNA (N1-methylpseudouridine)
EVERG500P: 500 µg EverGreen™️ mRNA (N1-methylpseudouridine)
EVERG1000P: 1 mg EverGreen™️ mRNA (N1-methylpseudouridine)
EVERG50M: 50 µg EverGreen™️ mRNA (5-methoxyuridine)
EVERG100M: 100 µg EverGreen™️ mRNA (5-methoxyuridine)
EVERG500M: 500 µg EverGreen™️ mRNA (5-methoxyuridine)
EVERG1000M: 1 mg EverGreen™️ mRNA (5-methoxyuridine)
(To pay with a purchase order, please send your purchase order to sales@messenger.bio.)
We are excited to release EverGreen™️ Fluorescent Protein mRNA, a precisely engineered mRNA that is 4-6 times brighter and almost 2x more photostable than an already optimized EGFP mRNA.
We developed EverGreen™️ mRNA by fine tuning the key determinants of EGFP mRNA expression, including the 5’ and 3’ UTRs as well as the protein-coding region itself.
EverGreen™️ mRNA includes a Cap1 structure (biologically superior to Cap0), a highly expressing 5’ UTR and highly stable 3’ UTR, a 100-nt poly(A) tail, and a codon-optimized open reading frame. Choose from unmodified or modified (N1-methylpseudouridine or 5-methoxyuridine) bases. Both types of modified bases have been shown to enhance protein expression in vitro and in vivo.
Our robust purification process removes dsRNA contaminants and other reaction components, generating highly pure mRNA that is capable of inducing very high levels of protein expression in diverse cell types, while causing minimal cellular innate immune activation (virtually undetectable).
As with all our mRNAs, this EverGreen™️ mRNA comes ready-to-use in a 1 mM sodium citrate buffer (pH 6.5), which is ideal for preserving RNA stability and extending shelf-life. Just complex with your favorite transfection reagent and you’re ready to go. Alternatively, speak to us about our ready-to-transfect LNP formulations.
If you would like to request a different amount of mRNA than what is listed on our website, or a different type of modified base, or any more information about our EverGreen™️ mRNA, send us an email at hello@messenger.bio and we’ll be happy to help.
For Research Use Only. See Terms of Sale for more.
