MS2-p65-HSF1 mRNA
Available in 3 options:
Naked mRNA (price displayed below)
mRNA + LNP (ready to transfect)*
mRNA + LNP mixture (DIY assembly of LNPs)*
*For pricing contact hello@messenger.bio
MSII50U: 50 µg MS2-p65-HSF1 mRNA (unmodified)
MSII100U: 100 µg MS2-p65-HSF1 mRNA (unmodified)
MSII500U: 500 µg MS2-p65-HSF1 mRNA (unmodified)
MSII1000U: 1 mg MS2-p65-HSF1 mRNA (unmodified)
MSII50P: 50 µg MS2-p65-HSF1 mRNA (N1-methylpseudouridine)
MSII100P: 100 µg MS2-p65-HSF1 mRNA (N1-methylpseudouridine)
MSII500P: 500 µg MS2-p65-HSF1 mRNA (N1-methylpseudouridine)
MSII1000P: 1 mg MS2-p65-HSF1 mRNA (N1-methylpseudouridine)
MSII50M: 50 µg MS2-p65-HSF1 mRNA (5-methoxyuridine)
MSII100M: 100 µg MS2-p65-HSF1 mRNA (5-methoxyuridine)
MSII500M: 500 µg MS2-p65-HSF1 mRNA (5-methoxyuridine)
MSII1000M: 1 mg MS2-p65-HSF1 mRNA (5-methoxyuridine)
(To pay with a purchase order, please send your purchase order to sales@messenger.bio)
Messenger Bio's MS2-p65-HSF1 mRNA is used to enhance your CRISPRa (dCas9-VP64) system. The MS2 domain specifically binds to an aptamer (sequence within your sgRNA - for design assistance contact us) and it can dramatically increase the effectiveness of your system.
We developed MS2-p65-HSF1 mRNA by fine-tuning the key determinants of MS2-p65-HSF1 mRNA expression, including the 5’ and 3’ UTRs as well as the protein-coding region itself.
MS2-p65-HSF1 mRNA includes a Cap1 structure (biologically superior to Cap0), a highly expressing 5’ UTR and highly stable 3’ UTR, a 100-nt poly(A) tail, and a codon-optimized open reading frame. Choose from unmodified or modified (N1-methylpseudouridine or 5-methoxyuridine) bases. Both types of modified bases have been shown to enhance protein expression in vitro and in vivo.
Our robust purification process removes dsRNA contaminants and other reaction components, generating highly pure mRNA that is capable of inducing very high levels of protein expression in diverse cell types, while causing minimal cellular innate immune activation (virtually undetectable).
As with all our mRNAs, this MS2-p65-HSF1 mRNA comes ready-to-use in a 1 mM sodium citrate buffer (pH 6.5), which is ideal for preserving RNA stability and extending shelf-life. Just complex with your favorite transfection reagent, and you’re ready to go. Alternatively, speak to us about our ready-to-transfect LNP formulations.
If you would like to request a different amount of mRNA than what is listed on our website, or a different type of modified base, or any more information about our MS2-p65-HSF1 mRNA, send us an email at hello@messenger.bio, and we’ll be happy to help.
For Research Use Only. See Terms of Sale for more.
