Prime Editing mRNA

Available as mRNA-LNP

(To pay with a purchase order, please send your purchase order to sales@messenger.bio.)

PE (Prime Editing) mRNA is a powerful new gene editing technology that can be used to specifically edit short sequences of DNA including: additions, deletions and substitutions. PE is more precise than traditional gene editing methods, such as CRISPR-Cas9, because it can target single-nucleotide changes without causing double-strand breaks. This makes it a powerful tool for correcting genetic defects and developing new therapies for diseases. We have multiple variants of the Prime Editing system for you to try; PE2, PE6 and PEmax.

Our PE mRNA incorporates a Cap1 structure (biologically superior to Cap0), a highly expressing 5’ UTR, a highly stable 3’ UTR, a 100-nt poly(A) tail, and a codon-optimized open reading frame. Choose from unmodified or modified (N1-methylpseudouridine or 5-methoxyuridine) bases. Both types of modified bases have been shown to enhance protein expression in vitro and in vivo.

Our robust purification process eliminates dsRNA contaminants and other reaction components, resulting in highly pure mRNA capable of inducing very high protein expression levels in diverse cell types, while causing almost no innate immune activation.

Like all our mRNA, our PE mRNA comes in a ready-to-use 1 mM sodium citrate buffer (pH 6.5). This buffer ensures optimal RNA stability and shelf-life. Simply complex with your preferred transfection reagent and you’re ready to go. Alternatively, speak to us about our ready-to-transfect mRNA-LNP formulations.

We understand that every research project is unique. If you need help with mRNA design/production or require a different mRNA quantity, a specific type of modified base, or further information about our PE mRNA, send us an email at hello@messenger.bio and we’ll be happy to help.

For Research Use Only. See Terms of Sale for more.